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mem against e coli atcc baa 2523  (ATCC)


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    ATCC mem against e coli atcc baa 2523
    Mem Against E Coli Atcc Baa 2523, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mem against e coli atcc baa 2523  (ATCC)
    95
    ATCC mem against e coli atcc baa 2523
    Mem Against E Coli Atcc Baa 2523, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    e coli bl21  (Thermo Fisher)
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    ( A ) EMSAs of the indicated SFL and SFL variants with the 55-nt −1 PRF-short RNA. Increasing protein concentrations used in EMSA are indicated on top of the gels. Representative of three independent experiments. ( B ) SDS-PAGE analysis of purified insect cell–expressed SFL, <t>E.</t> <t>coli</t> –expressed SFL, and insect cell–expressed SFL mutants S249A, T250A, T253A, S256A, S249D, T250D, T253D, and S256D. ( C to G ) BLI titrations of SFL mutants binding with −1 PRF-short RNA. Biotinylated −1 PRF-short RNAs were immobilized on SA biosensors for binding with SFL in solutions with a concentration series indicated on the top right. Binding kinetic parameters K D , k on , and k off are shown on top of each graph. Dashed lines are the fitting of the experimental data. Representative of two independent experiments. (C) Mutants S249A and S249D. (D) Mutants T250A and T250D. (E) Mutants T253A and T253D. (F) Mutants S256A and S256D. (G) Nonphosphorylated SFL expressed from E. coli . ( H ) Histogram illustrating the comparison of K D values of WT SFL and that of SFL mutants from the fitting of representative datasets. Error bars represent the fitting-derived K D error. ( I ) Western blot analyses of insect cell–expressed SFL alone, SFL treated with ALP of different enzyme units (2.5 and 5.0 U), and E. coli –expressed SFL; the samples were stained with SFL pAb or pan phosphor-Ser/Thr mAb. Representative of two independent experiments.
    E Coli Bl21, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    osteogenic induction medium  (MedChemExpress)
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    ( A ) EMSAs of the indicated SFL and SFL variants with the 55-nt −1 PRF-short RNA. Increasing protein concentrations used in EMSA are indicated on top of the gels. Representative of three independent experiments. ( B ) SDS-PAGE analysis of purified insect cell–expressed SFL, <t>E.</t> <t>coli</t> –expressed SFL, and insect cell–expressed SFL mutants S249A, T250A, T253A, S256A, S249D, T250D, T253D, and S256D. ( C to G ) BLI titrations of SFL mutants binding with −1 PRF-short RNA. Biotinylated −1 PRF-short RNAs were immobilized on SA biosensors for binding with SFL in solutions with a concentration series indicated on the top right. Binding kinetic parameters K D , k on , and k off are shown on top of each graph. Dashed lines are the fitting of the experimental data. Representative of two independent experiments. (C) Mutants S249A and S249D. (D) Mutants T250A and T250D. (E) Mutants T253A and T253D. (F) Mutants S256A and S256D. (G) Nonphosphorylated SFL expressed from E. coli . ( H ) Histogram illustrating the comparison of K D values of WT SFL and that of SFL mutants from the fitting of representative datasets. Error bars represent the fitting-derived K D error. ( I ) Western blot analyses of insect cell–expressed SFL alone, SFL treated with ALP of different enzyme units (2.5 and 5.0 U), and E. coli –expressed SFL; the samples were stained with SFL pAb or pan phosphor-Ser/Thr mAb. Representative of two independent experiments.
    Osteogenic Induction Medium, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    shig 3 3 shiga toxin producing e coli stec atcc baa2217 medium 7 5x103  (ATCC)
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    ATCC shig 3 3 shiga toxin producing e coli stec atcc baa2217 medium 7 5x103
    ( A ) EMSAs of the indicated SFL and SFL variants with the 55-nt −1 PRF-short RNA. Increasing protein concentrations used in EMSA are indicated on top of the gels. Representative of three independent experiments. ( B ) SDS-PAGE analysis of purified insect cell–expressed SFL, <t>E.</t> <t>coli</t> –expressed SFL, and insect cell–expressed SFL mutants S249A, T250A, T253A, S256A, S249D, T250D, T253D, and S256D. ( C to G ) BLI titrations of SFL mutants binding with −1 PRF-short RNA. Biotinylated −1 PRF-short RNAs were immobilized on SA biosensors for binding with SFL in solutions with a concentration series indicated on the top right. Binding kinetic parameters K D , k on , and k off are shown on top of each graph. Dashed lines are the fitting of the experimental data. Representative of two independent experiments. (C) Mutants S249A and S249D. (D) Mutants T250A and T250D. (E) Mutants T253A and T253D. (F) Mutants S256A and S256D. (G) Nonphosphorylated SFL expressed from E. coli . ( H ) Histogram illustrating the comparison of K D values of WT SFL and that of SFL mutants from the fitting of representative datasets. Error bars represent the fitting-derived K D error. ( I ) Western blot analyses of insect cell–expressed SFL alone, SFL treated with ALP of different enzyme units (2.5 and 5.0 U), and E. coli –expressed SFL; the samples were stained with SFL pAb or pan phosphor-Ser/Thr mAb. Representative of two independent experiments.
    Shig 3 3 Shiga Toxin Producing E Coli Stec Atcc Baa2217 Medium 7 5x103, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    magicmedia tm e coli expression medium  (Thermo Fisher)
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    ( A ) EMSAs of the indicated SFL and SFL variants with the 55-nt −1 PRF-short RNA. Increasing protein concentrations used in EMSA are indicated on top of the gels. Representative of three independent experiments. ( B ) SDS-PAGE analysis of purified insect cell–expressed SFL, <t>E.</t> <t>coli</t> –expressed SFL, and insect cell–expressed SFL mutants S249A, T250A, T253A, S256A, S249D, T250D, T253D, and S256D. ( C to G ) BLI titrations of SFL mutants binding with −1 PRF-short RNA. Biotinylated −1 PRF-short RNAs were immobilized on SA biosensors for binding with SFL in solutions with a concentration series indicated on the top right. Binding kinetic parameters K D , k on , and k off are shown on top of each graph. Dashed lines are the fitting of the experimental data. Representative of two independent experiments. (C) Mutants S249A and S249D. (D) Mutants T250A and T250D. (E) Mutants T253A and T253D. (F) Mutants S256A and S256D. (G) Nonphosphorylated SFL expressed from E. coli . ( H ) Histogram illustrating the comparison of K D values of WT SFL and that of SFL mutants from the fitting of representative datasets. Error bars represent the fitting-derived K D error. ( I ) Western blot analyses of insect cell–expressed SFL alone, SFL treated with ALP of different enzyme units (2.5 and 5.0 U), and E. coli –expressed SFL; the samples were stained with SFL pAb or pan phosphor-Ser/Thr mAb. Representative of two independent experiments.
    Magicmedia Tm E Coli Expression Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ( A ) EMSAs of the indicated SFL and SFL variants with the 55-nt −1 PRF-short RNA. Increasing protein concentrations used in EMSA are indicated on top of the gels. Representative of three independent experiments. ( B ) SDS-PAGE analysis of purified insect cell–expressed SFL, <t>E.</t> <t>coli</t> –expressed SFL, and insect cell–expressed SFL mutants S249A, T250A, T253A, S256A, S249D, T250D, T253D, and S256D. ( C to G ) BLI titrations of SFL mutants binding with −1 PRF-short RNA. Biotinylated −1 PRF-short RNAs were immobilized on SA biosensors for binding with SFL in solutions with a concentration series indicated on the top right. Binding kinetic parameters K D , k on , and k off are shown on top of each graph. Dashed lines are the fitting of the experimental data. Representative of two independent experiments. (C) Mutants S249A and S249D. (D) Mutants T250A and T250D. (E) Mutants T253A and T253D. (F) Mutants S256A and S256D. (G) Nonphosphorylated SFL expressed from E. coli . ( H ) Histogram illustrating the comparison of K D values of WT SFL and that of SFL mutants from the fitting of representative datasets. Error bars represent the fitting-derived K D error. ( I ) Western blot analyses of insect cell–expressed SFL alone, SFL treated with ALP of different enzyme units (2.5 and 5.0 U), and E. coli –expressed SFL; the samples were stained with SFL pAb or pan phosphor-Ser/Thr mAb. Representative of two independent experiments.
    Magicmediatm E Coli Expression Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mem against e coli atcc 25922  (ATCC)
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    ATCC mem against e coli atcc 25922
    ( A ) EMSAs of the indicated SFL and SFL variants with the 55-nt −1 PRF-short RNA. Increasing protein concentrations used in EMSA are indicated on top of the gels. Representative of three independent experiments. ( B ) SDS-PAGE analysis of purified insect cell–expressed SFL, <t>E.</t> <t>coli</t> –expressed SFL, and insect cell–expressed SFL mutants S249A, T250A, T253A, S256A, S249D, T250D, T253D, and S256D. ( C to G ) BLI titrations of SFL mutants binding with −1 PRF-short RNA. Biotinylated −1 PRF-short RNAs were immobilized on SA biosensors for binding with SFL in solutions with a concentration series indicated on the top right. Binding kinetic parameters K D , k on , and k off are shown on top of each graph. Dashed lines are the fitting of the experimental data. Representative of two independent experiments. (C) Mutants S249A and S249D. (D) Mutants T250A and T250D. (E) Mutants T253A and T253D. (F) Mutants S256A and S256D. (G) Nonphosphorylated SFL expressed from E. coli . ( H ) Histogram illustrating the comparison of K D values of WT SFL and that of SFL mutants from the fitting of representative datasets. Error bars represent the fitting-derived K D error. ( I ) Western blot analyses of insect cell–expressed SFL alone, SFL treated with ALP of different enzyme units (2.5 and 5.0 U), and E. coli –expressed SFL; the samples were stained with SFL pAb or pan phosphor-Ser/Thr mAb. Representative of two independent experiments.
    Mem Against E Coli Atcc 25922, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    e. coli chromogenic medium hb7001  (Haibo Inc)
    90
    Haibo Inc e. coli chromogenic medium hb7001
    ( A ) EMSAs of the indicated SFL and SFL variants with the 55-nt −1 PRF-short RNA. Increasing protein concentrations used in EMSA are indicated on top of the gels. Representative of three independent experiments. ( B ) SDS-PAGE analysis of purified insect cell–expressed SFL, <t>E.</t> <t>coli</t> –expressed SFL, and insect cell–expressed SFL mutants S249A, T250A, T253A, S256A, S249D, T250D, T253D, and S256D. ( C to G ) BLI titrations of SFL mutants binding with −1 PRF-short RNA. Biotinylated −1 PRF-short RNAs were immobilized on SA biosensors for binding with SFL in solutions with a concentration series indicated on the top right. Binding kinetic parameters K D , k on , and k off are shown on top of each graph. Dashed lines are the fitting of the experimental data. Representative of two independent experiments. (C) Mutants S249A and S249D. (D) Mutants T250A and T250D. (E) Mutants T253A and T253D. (F) Mutants S256A and S256D. (G) Nonphosphorylated SFL expressed from E. coli . ( H ) Histogram illustrating the comparison of K D values of WT SFL and that of SFL mutants from the fitting of representative datasets. Error bars represent the fitting-derived K D error. ( I ) Western blot analyses of insect cell–expressed SFL alone, SFL treated with ALP of different enzyme units (2.5 and 5.0 U), and E. coli –expressed SFL; the samples were stained with SFL pAb or pan phosphor-Ser/Thr mAb. Representative of two independent experiments.
    E. Coli Chromogenic Medium Hb7001, supplied by Haibo Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ( A ) EMSAs of the indicated SFL and SFL variants with the 55-nt −1 PRF-short RNA. Increasing protein concentrations used in EMSA are indicated on top of the gels. Representative of three independent experiments. ( B ) SDS-PAGE analysis of purified insect cell–expressed SFL, E. coli –expressed SFL, and insect cell–expressed SFL mutants S249A, T250A, T253A, S256A, S249D, T250D, T253D, and S256D. ( C to G ) BLI titrations of SFL mutants binding with −1 PRF-short RNA. Biotinylated −1 PRF-short RNAs were immobilized on SA biosensors for binding with SFL in solutions with a concentration series indicated on the top right. Binding kinetic parameters K D , k on , and k off are shown on top of each graph. Dashed lines are the fitting of the experimental data. Representative of two independent experiments. (C) Mutants S249A and S249D. (D) Mutants T250A and T250D. (E) Mutants T253A and T253D. (F) Mutants S256A and S256D. (G) Nonphosphorylated SFL expressed from E. coli . ( H ) Histogram illustrating the comparison of K D values of WT SFL and that of SFL mutants from the fitting of representative datasets. Error bars represent the fitting-derived K D error. ( I ) Western blot analyses of insect cell–expressed SFL alone, SFL treated with ALP of different enzyme units (2.5 and 5.0 U), and E. coli –expressed SFL; the samples were stained with SFL pAb or pan phosphor-Ser/Thr mAb. Representative of two independent experiments.

    Journal: Science Advances

    Article Title: Phosphorylation of shiftless is important for inhibiting the programmed −1 ribosomal frameshift

    doi: 10.1126/sciadv.adw7471

    Figure Lengend Snippet: ( A ) EMSAs of the indicated SFL and SFL variants with the 55-nt −1 PRF-short RNA. Increasing protein concentrations used in EMSA are indicated on top of the gels. Representative of three independent experiments. ( B ) SDS-PAGE analysis of purified insect cell–expressed SFL, E. coli –expressed SFL, and insect cell–expressed SFL mutants S249A, T250A, T253A, S256A, S249D, T250D, T253D, and S256D. ( C to G ) BLI titrations of SFL mutants binding with −1 PRF-short RNA. Biotinylated −1 PRF-short RNAs were immobilized on SA biosensors for binding with SFL in solutions with a concentration series indicated on the top right. Binding kinetic parameters K D , k on , and k off are shown on top of each graph. Dashed lines are the fitting of the experimental data. Representative of two independent experiments. (C) Mutants S249A and S249D. (D) Mutants T250A and T250D. (E) Mutants T253A and T253D. (F) Mutants S256A and S256D. (G) Nonphosphorylated SFL expressed from E. coli . ( H ) Histogram illustrating the comparison of K D values of WT SFL and that of SFL mutants from the fitting of representative datasets. Error bars represent the fitting-derived K D error. ( I ) Western blot analyses of insect cell–expressed SFL alone, SFL treated with ALP of different enzyme units (2.5 and 5.0 U), and E. coli –expressed SFL; the samples were stained with SFL pAb or pan phosphor-Ser/Thr mAb. Representative of two independent experiments.

    Article Snippet: E. coli BL21 (DE3; Thermo Fisher Scientific, EC0114) cells were grown in Luria-Bertani (LB) medium.

    Techniques: SDS Page, Purification, Binding Assay, Concentration Assay, Comparison, Derivative Assay, Western Blot, Staining